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Leishrisk, Bridging Research and Leishmanianis Control

Leishrisk, Bridging Research and Leishmanianis Control

Kaladrug-R  standard operating protocols

 


| introduction | overview workpackages | Kaladrug-R SOPs | agenda | publications | contacts |


 

available SOPs: monitoring tools and SOPSclinical sample SOPs - laboratory SOPs - management SOPs

 

All these SOPs are provided as is, and the protocols presented have been designed specifically for the Kaladrug-R project. They are freely availale for use by external parties, but the Kaladrug-R consortium accepts no liability for any unexpected results or outcome. You can contact mentioned authors for more information.

If you choose to use any of the Kaladrug-R SOPs, we would appreciate that you refer to this website in your publication.

A. VL Treatment Outcome Monitoring Tools: Register, Report Forms and Manuals ready for use

1. Retrospective Quarterly Cohort Monitoring for Visceral Leishmaniasis - coming soon

2. SOP Daily Record Register for Kala-Azar - download PDF

3. Kala-Azar Daily Record Register - download Excel version

4. SOP Retrospective Cohort Analysis - download PDF

5. Blanc Quarterly Report Form - download Word version

6. Example Quarterly Report Form -download Word version

B. Clinical sample SOPs: WHEN and HOW should samples be collected? (download overview doc)

SOP #A - Nepalese L. donovani isolation from bone marrow/spleen aspirates - download PDF    
SOP #B - Bone marrow/spleen aspirates for PCR and DNA extraction - download PDF    
SOP #C - Blood sample and filterpaper for PCR - download PDF    
SOP #D - Isolation of parasites from PKDL tissue lesions download PDF
SOP #E - Labelling clinical samples BPKIHS - download PDF      
SOP #F - Labelling clinical samples BHU/KAMRC - download PDF      
SOP #G - Indian L. donovani isolation from spleen aspirates (BHU method) - download PDF      
SOP #H - Indian L. donovani isolation from blood samples (BHU method) - download PDF          

  

B. laboratory SOPs

SOP #1a - Promastigote DNA extraction at ITMA for sequencing - download PDF
SOP #1b - Promastigote DNA extraction outside ITMA for sequencing - download PDF
SOP #2 - DNA preparation for Illumnia sequencing - in preparation
SOP #3 - L. donovani specific PCR (cpb-F & ITS1) - download PDF
SOP #4 - kDNA PCR-RFLP for L. donovani - download PDF
SOP #5 - Cryopreservation L. donovani promastigote cultures - download PDF
SOP #6 - Development of miltefosine resistant L. donovani (promastigotes) - in preparation
SOP #7 - Development of paromomycin resistant L. donovani (promastigotes) - in preparation
SOP #8 - SSG susceptibility testing L. donovani intracellular amastigotes - download PDF
SOP #9 - Miltefosine susceptibility testing L. donovani intracellular amastigotes - download PDF
SOP #10 - Cloning of L. donovani isolates - download PDF
SOP #11 - Culturing reference L. donovani strains - download PDF
SOP #12 - Paromomycin susceptibility testing L. donovani intracellular amastigotes - in preparation
SOP #13 - Labelling of L. donovani strains for lab use - download PDF
SOP #14 - Miltefosine susceptibility testing L. donovani promastigotes - in preparation
SOP #15 - Paromomycin susceptibility testing L. donovani promastigotes - in preparation
NEW  SOP #16 - L. donovani diagnostic PCR (SSU) - download PDF
NEW  SOP #17 - Preparation of stock solutions of reference drugs for in vitro application - download PDF
NEW  SOP #18 - Simple method and tool for calculation IC50-values - download PDF

C. management SOPs

SOP I-A - clinical database BPKIHS (Nepal) - data collection and data entry for KA patients - download PDF      
SOP I-B - clinical database KAMRC (India) - data collection and data entry for KA patients - in preparation
SOP II-A - BPKIHS sample database - data management, database input & monitoring - download PDF      
SOP II-B - BHU sample database - data management, database input & monitoring - download PDF      
SOP III - communication and contact details - download PDF
SOP IV - data management at ITMA - in preparation

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